Search results for "cellular resolution"
showing 3 items of 3 documents
Direct activation of zebrafish neurons by ultrasonic stimulation revealed by whole CNS calcium imaging
2020
Abstract Objective. Ultrasounds (US) use in neural engineering is so far mainly limited to ablation through high intensity focused ultrasound, but interesting preliminary results show that low intensity low frequency ultrasound could be used instead to modulate neural activity. However, the extent of this modulatory ability of US is still unclear, as in in vivo studies it is hard to disentangle the contribution to neural responses of direct activation of the neuron by US stimulation and indirect activation due either to sensory response to mechanical stimulation associated to US, or to propagation of activity from neighboring areas. Here, we aim to show how to separate the three effects and…
Multisite field potential recordings and analysis of the impulse propagation pattern in cardiac cells culture
2007
To provide further insights into the impulse propagation between cardiac myocytes, we performed multiparametric studies of excitation spread with cellular resolution in confluent monolayers of cultured cardiomyocytes (CM). Simultaneous paired intracellular recordings of action potentials in two individual CM revealed slight periodic spontaneous advances/delays in the interspike time lag. Multisite field potential recordings performed with microelectrode arrays (MEA) confirmed random and iterative cycle-to-cycle changes in the direction of excitation spread. These local spontaneous variations in the cardiac impulse propagation pathways may be a safety process protecting against microscopical…
Cardiac arrhythmias induced by an electrical stimulation at a cellular level
2008
To provide insights into the impulse propagation between cardiac myocytes, we performed studies of excitation spread with cellular resolution in confluent monolayers of cultured cardiomyocytes (CM). Multisite field potentials have been recorded using microelectrode arrays (MEA) technology in a basal condition and in proarrhythmic conditions induced by a high frequency electrical stimulation. The in vitro observation of spiral waves opens a new way to test the anti-arrhythmic drugs or strategies at cellular level.